ptau s396  (Meso Scale Diagnostics LLC)

Journal: Npj Dementia

Article Title: Post-symptomatic NLRP3 inhibition rescues cognitive impairment and mitigates amyloid and tau driven neurodegeneration

doi: 10.1038/s44400-025-00011-5

Figure Lengend Snippet: Representative immunofluorescence imaging for pTau S396 (red) and DAPI (blue) in cortex ( A ) and hippocampus ( C ). Scale bars, 50 um for ( A ), 100 um for ( C ). B , D Quantification of pTau S396 mean area in cortex and hippocampus respectively. Data are presented as mean ± SEM. ** p < 0.01, *** p < 0.0001.

Article Snippet: Sections were rinsed in PBS, blocked in 3% bovine serum albumin/0.2% Triton X-100/PBS for 1 h, and incubated with primary antibodies in blocking buffer overnight at 4 °C (1:200 goat anti-Iba1 [Novus Biologicals, Centennial, CO], 1:500 mouse anti-Aβ 82E1 MOAB [Immuno-Biological Laboratories, Inc., Spring Lake Park, MN], 1:200 rabbit anti-pTau S396 [Cell Signaling, Danvers, MA], 1:200 mouse anti-NeuN [Cell Signaling]).

Techniques: Immunofluorescence, Imaging

Journal: Frontiers in Neuroscience

Article Title: Modeling Alzheimer’s disease related phenotypes in the Ts65Dn mouse: impact of age on Aβ, Tau, pTau, NfL, and behavior

doi: 10.3389/fnins.2023.1202208

Figure Lengend Snippet: Total Tau and pTau S396 protein level in Ts65Dn and 2N mouse brain total homogenate and insoluble fraction at different ages by Meso Scale Discovery and WB. (A) Schematic representation of the fractions used for evaluation. The total homogenate was used for measuring total Tau and pTau levels. P3-SinT was used for the Sarkosyl insoluble Tau fraction. (B) Total Tau measured by Meso Scale Discovery. Age had a significant effect on levels of total Tau ( p < 0.0001). (C) Total Tau as measured by WB. Age had a significant effect ( p = 0.0218) as did genotype ( p = 0.0218). (D) pTau S396 protein level in total brain homogenates of 2N and TS65Dn mice at different ages as measured by Meso Scale Discovery. Age ( p < 0.0001) and genotype ( p = 0.0228) were significant factors for pTau S396 levels. (E) WB evaluation of pTau S396 protein level in total brain homogenates of 2N and TS65Dn mice at different ages measured. Age ( p < 0.0001) was a significant factor for pTau S396 levels. The concentrations of pTauS396 obtained by Meso Scale Discovery were normalized on the total protein concentration, while the results obtained by WB were normalized on the total protein concentration and expressed as the corrected area. (F) pTau S396 protein level in Sarkosyl-insoluble fraction of the 13- and 16-month-old cohort measured by WB. ANOVA indicated a significant effect of genotype ( p = 0.0066), age ( p = 0.0024), and interaction of age and genotype ( p = 0.0066). (G) pTau S396 protein level in Sarkosyl-insoluble fraction of the 16-month-old cohort measured by Meso Scale Discovery. Results are expressed as individual values and mean ± SD.

Article Snippet: Post hoc Tukey’s multiple comparisons tests identified significant increases in pTau S396 levels measured by Meso Scale Discovery in Ts65Dn mice at 10 ( p < 0.0001), 13 ( p < 0.0001), and 16 ( p < 0.0001) months of age compared to 7 months of age, and at 16 months of age compared to 10 months of age ( p = 0.0053).

Techniques: Protein Concentration